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Allosteric Molecular Beacons for Sensitive Detection of Nucleic Acids, Proteins, and Small Molecules in Complex Biological Samples
Author(s) -
Song Yanling,
Cui Liang,
Wu Jie,
Zhang Weiting,
Zhang Wei Yun,
Kang Huaizhi,
Yang Chaoyong James
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201101353
Subject(s) - molecular beacon , allosteric regulation , nucleic acid , streptavidin , chemistry , aptamer , nucleic acid quantitation , small molecule , biomolecule , biophysics , combinatorial chemistry , biochemistry , biology , biotin , dna , oligonucleotide , enzyme , microbiology and biotechnology
Beacon of light! A new type of probe called an allosteric molecular beacon (aMB) has been designed for highly sensitive detection of nucleic acids, proteins, and small molecules. The aMB is designed in such way that target binding will activate its binding affinity to streptavidin microbeads for probe signal enrichment, noise reduction, and signal readout, which enables ultrasensitive target detection in complex biological samples (see scheme).