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A New Ratiometric Fluorescent Probe for Detection of Fe 2+ with High Sensitivity and Its Intracellular Imaging Applications
Author(s) -
Li Ping,
Fang Libo,
Zhou Hui,
Zhang Wen,
Wang Xu,
Li Na,
Zhong Hongbo,
Tang Bo
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201101327
Subject(s) - fluorophore , fluorescence , intracellular , sensitivity (control systems) , chemistry , fluorescence lifetime imaging microscopy , biophysics , analytical chemistry (journal) , optics , physics , biology , biochemistry , chromatography , electronic engineering , engineering
Good sense : A highly sensitive and selective ratiometric fluorescent probe for Fe 2+ was developed by linking an Fe 2+ ‐insensitive fluorophore to an Fe 2+ ‐sensitive reporter–fluorophore fragment. The fluorescence intensity ratio increased linearly with the concentration of Fe 2+ (see scheme). The probe was applied to the ratiometric imaging of intracellular and adscititious fluctuations of Fe 2+ in live HL‐7702 and HepG2 cells.