Premium
Crystal Structures and Repair Studies Reveal the Identity and the Base‐Pairing Properties of the UV‐Induced Spore Photoproduct DNA Lesion
Author(s) -
Heil Korbinian,
Kneuttinger Andrea Christa,
Schneider Sabine,
Lischke Ulrike,
Carell Thomas
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201100177
Subject(s) - phosphodiester bond , lesion , dna , oligonucleotide , dna damage , base pair , biology , dna repair , nucleotide excision repair , biochemistry , chemistry , gene , rna , medicine , pathology
UV light is one of the major causes of DNA damage. In spore DNA, due to an unusual packing of the genetic material, a special spore photoproduct lesion (SP lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (Spl), a radical S ‐adenosylmethionine (SAM) enzyme. We report here the synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone. The oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enzyme as a template to clarify the configuration of the SP lesion and to provide information about the base‐pairing properties of the lesion. The structural analysis together with repair studies allowed us to clarify the identity of the preferentially repaired lesion diastereoisomer.