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Detection of Single Nucleotide Polymorphisms by a Gold Nanowire‐on‐Film SERS Sensor Coupled with S1 Nuclease Treatment
Author(s) -
Yoo Seung Min,
Kang Taejoon,
Kim Bongsoo,
Lee Sang Yup
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201003372
Subject(s) - nuclease , single nucleotide polymorphism , snp , raman scattering , dna , materials science , nucleotide , biology , genetics , nanotechnology , computational biology , raman spectroscopy , gene , genotype , optics , physics
Single nucleotide polymorphisms (SNPs) can serve as important biomarkers for genetic diseases, for which accurate detection of SNPs is essential for early diagnosis. We have developed a novel SNP sensor by combining a Au nanowire‐on‐film surface‐enhanced Raman scattering (SERS) platform with S1 nuclease reaction. The combined sensor system provides reproducible SERS signals only in the presence of perfectly matched target DNAs, to probe DNAs as a result of single‐stranded DNA‐specific degradation by S1 nuclease. Furthermore, point mutations in DNA causing Wilson disease and Avellino corneal dystrophy were successfully identified by this sensor, thereby indicating its practical ability to diagnose genetic diseases.