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Catalyst‐Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu‐Free Click Chemistry
Author(s) -
Jølck Rasmus I.,
Sun Honghao,
Berg Rolf H.,
Andresen Thomas L.
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201003131
Subject(s) - fluorophore , click chemistry , ligand (biochemistry) , nanoparticle , combinatorial chemistry , chemistry , in situ , nanotechnology , fluorescence , materials science , organic chemistry , biochemistry , receptor , physics , quantum mechanics
A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3‐azido‐modifed RGD‐peptides to PEGylated liposomes was achieved by using Cu‐free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification.