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Dynamic‐Light‐Scattering‐Based Sequence‐Specific Recognition of Double‐Stranded DNA with Oligonucleotide‐Functionalized Gold Nanoparticles
Author(s) -
Miao XiangMin,
Xiong Cen,
Wang WeiWei,
Ling LianSheng,
Shuai XinTao
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201003010
Subject(s) - oligonucleotide , dynamic light scattering , dna , colloidal gold , detection limit , nanoparticle , microbiology and biotechnology , sequence (biology) , materials science , chemistry , biophysics , nanotechnology , chromatography , biology , biochemistry
An ultrasensitive and simple dynamic‐light‐scattering (DLS) assay for the sequence‐specific recognition of double‐stranded DNA (dsDNA) was developed based on detection of the average diameter change of Au nanoparticle (AuNP) probes modified with oligonucleotides 5′‐TTTCTCTTCCTT‐ CTCTTC‐(T) 12 ‐SH‐3′ (Oligo 1) and 5′‐TTCTTTCTTTTCC‐(T) 12 ‐ SH‐3′ (Oligo 2). The target dsDNA was composed of two complementary oligonucleotides: 5′‐AAAGAGAAGGAAGAGAAGAAGAAAGAAAAGG‐3′ (Oligo 3) and 3′‐TTTCTCTTCCTTCTCTTCTTCTTTCTTTTCC‐5′ (Oligo 4). Hybridization of the two AuNPs–Oligo probes with the target dsDNA induced aggregation of the target dsDNA by forming triplex DNA, which accordingly increased the average diameter. This diameter change could then be detected by DLS. The average diameter was proportional to the target dsDNA concentration over the range from 593 f M to 40 p M , with a detection limit of 593 f M . Moreover, the assay had good sequence specificity for the target dsDNA.