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Aptamer/Nanoparticle‐Based Sensitive, Multiplexed Electronic Coding of Proteins and Small Biomolecules through a Backfilling Strategy
Author(s) -
Qian Xiaoqing,
Xiang Yun,
Zhang Haixia,
Chen Ying,
Chai Yaqin,
Yuan Ruo
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201002585
Subject(s) - aptamer , biomolecule , analyte , multiplexing , nanoparticle , dna , lysozyme , conjugated system , nanotechnology , biosensor , chemistry , computational biology , materials science , microbiology and biotechnology , biochemistry , computer science , biology , chromatography , polymer , telecommunications , organic chemistry
Sensing large and small simultaneously : Sensitive and multiplexed analysis of lysozyme and ATP analytes is achieved through target‐induced release of aptamers from the DNA duplexes on a sensing surface, followed by backfilling hybridization of the resulting single‐stranded DNAs with aptamers conjugated to the electrochemically encoded nanoparticles (see graphic).