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Reversible DNA Condensation Induced by a Tetranuclear Nickel(II) Complex
Author(s) -
Dong Xindian,
Wang Xiaoyong,
He Yafeng,
Yu Zhen,
Lin Miaoxin,
Zhang Changli,
Wang Jing,
Song Yajie,
Zhang Yangmiao,
Liu Zhipeng,
Li Yizhi,
Guo Zijian
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201001457
Subject(s) - dna condensation , ethylenediaminetetraacetic acid , circular dichroism , dna , chemistry , crystallography , zeta potential , monoclinic crystal system , fluorescence spectroscopy , dynamic light scattering , chelation , fluorescence , materials science , nanoparticle , inorganic chemistry , crystal structure , biochemistry , nanotechnology , gene , transfection , physics , quantum mechanics
DNA condensing agents play a critical role in gene therapy. A tetranuclear nickel(II) complex, [Ni II 4 (L−2H)(H 2 O) 6 (CH 3 CH 2 OH) 2 ] ⋅ 6NO 3 (L=3,3',5,5'‐tetrakis{[(2‐hydroxyethyl)(pyridin‐2‐ylmethyl)amino]methyl}biphenyl‐4,4'‐diol), has been synthesized as a nonviral vector to induce DNA condensation. X‐ray crystallographic data indicate that the complex crystallizes in the monoclinic system with space group P 2 1 / n , a =10.291(9), b =24.15(2), c =13.896(11) Å, and β =98.175(13)°. The DNA condensation induced by the complex has been investigated by means of UV/Vis spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy, dynamic light scattering, atomic force microscopy, gel electrophoresis assay, and zeta potential analysis. The complex interacts strongly with DNA through electrostatic attraction and induces its condensation into globular nanoparticles at low concentration. The release of DNA from its compact state has been achieved using the chelator ethylenediaminetetraacetic acid (EDTA) for the first time. Other essential properties, such as DNA cleavage inactivity and biocompatibility, have also been examined in vitro. In general, the complex satisfies the requirements of a gene vector in all of these respects.