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Corozalic Acid: A Key Okadaic Acid Biosynthetic Precursor with Phosphatase Inhibition Activity
Author(s) -
Napolitano José G.,
Norte Manuel,
Fernández José Javier,
Hernández Daranas Antonio
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201001327
Subject(s) - okadaic acid , protein phosphatase 2 , phosphatase , dinoflagellate , docking (animal) , chemistry , biochemistry , acid phosphatase , toxin , protein phosphatase 1 , enzyme , stereochemistry , computational biology , biology , botany , medicine , nursing
Toxin precursors : Corozalic acid, a new Ser/Thr protein phosphatase 1 and 2A inhibitor that turned out to be a key metabolic intermediate, was isolated from cultures of the marine dinoflagellate Prorocentrum belizeanum. Detailed spectroscopic analysis and extensive computational calculations revealed its structure and conformational behavior. In addition, a comprehensive picture of the interactions of corozalic acid with PP1 and PP2A is proposed (see figure) on the basis of ab initio, docking and molecular dynamics calculations.