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A Lanthanide‐Complex‐Based Ratiometric Luminescent Probe Specific for Peroxynitrite
Author(s) -
Song Cuihong,
Ye Zhiqiang,
Wang Guilan,
Yuan Jingli,
Guan Yafeng
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201000528
Subject(s) - luminescence , lanthanide , aqueous solution , chemistry , luminophore , photochemistry , materials science , organic chemistry , optoelectronics , ion
A lanthanide‐complex‐based ratiometric luminescence probe specific for peroxynitrite (ONOO − ), 4′‐(2,4‐dimethoxyphenyl)‐2,2′:6′,2′′‐terpyridine‐6,6′′‐diyl]bis(methylenenitrilo)tetrakis(acetate)‐Eu 3+ /Tb 3+ ([Eu 3+ /Tb 3+ (DTTA)]), has been designed and synthesized. Both [Eu 3+ (DTTA)] and [Tb 3+ (DTTA)] are highly water soluble with large stability constants at ≈10 20 , and strongly luminescent with luminescence quantum yields of 10.0 and 9.9 %, respectively, and long luminescence lifetimes of 1.38 and 0.26 ms, respectively. It was found that the luminescence of [Tb 3+ (DTTA)] could be quenched by ONOO − rapidly and specifically in aqueous buffers, while that of [Eu 3+ (DTTA)] did not respond to the addition of ONOO − . Thus, by simply mixing [Eu 3+ (DTTA)] and [Tb 3+ (DTTA)] in an aqueous buffer, a ratiometric luminescence probe specific for time‐gated luminescence detection of ONOO − was obtained. The performance of [Tb 3+ (DTTA)] and [Eu 3+ /Tb 3+ (DTTA)] as the probes for luminescence imaging detection of ONOO − in living cells was investigated. The results demonstrated the efficacy and advantages of the new ratiometric luminescence probe for highly sensitive luminescence bioimaging application.