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Localized Deposition of Au Nanoparticles by Direct Electron Transfer through Cellobiose Dehydrogenase
Author(s) -
Malel Esteban,
Ludwig Roland,
Gorton Lo,
Mandler Daniel
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201000453
Subject(s) - cellobiose dehydrogenase , electron transfer , chemistry , flavin adenine dinucleotide , photochemistry , flavin group , nicotinamide adenine dinucleotide , ferricyanide , heme , colloidal gold , hydroquinone , nanoparticle , cofactor , combinatorial chemistry , cellobiose , nanotechnology , inorganic chemistry , materials science , enzyme , organic chemistry , nad+ kinase , cellulase
Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome‐type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized deposition of gold on Pd substrates by CDH through DET and MET. For this purpose we exploited the advantage of scanning electrochemical microscopy (SECM) as a patterning tool. We first demonstrated that gold nanoparticles can be formed in homogenous solution. Then we showed that Au nanoparticles can also be locally formed and deposited on surfaces through DET at low pH and by MET at neutral pH using benzoquinone/hydroquinone as mediator.