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A Photo‐Cross‐Linking Strategy to Map Sites of Protein–Protein Interactions
Author(s) -
Chen Yihui,
Wu Yalin,
Henklein Peter,
Li Xiaoxu,
Hofmann Klaus Peter,
Nakanishi Koji,
Ernst Oliver P.
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201000441
Subject(s) - peptide , protein–protein interaction , azide , click chemistry , computer science , chemistry , g protein coupled receptor , computational biology , information retrieval , combinatorial chemistry , biochemistry , receptor , biology , organic chemistry
Clicked! Exemplified on a G‐protein‐coupled receptor (GPCR; see figure)/G‐protein model system, a specific protein–peptide interaction was analyzed by using a G‐protein‐derived peptide containing p ‐ethynylbenzoyl‐modified phenylalanine. Click chemistry with a biotin propyl azide probe enabled isolation of photo‐cross‐linked products and facilitated mass spectrometric analysis of the protein–peptide interaction sites.