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Programmed‐Assembly System Using DNA Jigsaw Pieces
Author(s) -
Endo Masayuki,
Sugita Tsutomu,
Katsuda Yousuke,
Hidaka Kumi,
Sugiyama Hiroshi
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200903057
Subject(s) - jigsaw , dna origami , dna , complementarity (molecular biology) , a dna , computer science , nanotechnology , mathematics , materials science , biology , genetics , mathematics education
A novel method for assembling multiple DNA origami structures has been developed by using designed 2D DNA origami rectangles, so‐called “DNA jigsaw pieces” that have sequence‐programmed connectors. Shape and sequence complementarity were introduced to the concavity and convex connectors in the DNA rectangles for selective connection with the help of nonselective π‐stacking interactions between the side edges of the DNA jigsaw piece structures. Single DNA jigsaw piece units were assembled into unidirectional nanostructures with the correct alignment and uniform orientation. Three and five different DNA jigsaw pieces were assembled into predesigned and ordered nanostructures in a programmed fashion. Finally, three‐, four‐, and five‐letter words have been displayed by using this programmed DNA jigsaw piece system.