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Poly(arginine)‐Selective Coprecipitation Properties of Self‐Assembling Apoferritin and Its Tb 3+ Complex: A New Luminescent Biotool for Sensing of Poly(arginine) and Its Protein Conjugates
Author(s) -
Tsukube Hiroshi,
Noda Yuki,
Shinoda Satoshi
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200902833
Subject(s) - luminescence , coprecipitation , arginine , aqueous solution , conjugate , dynamic light scattering , chemistry , amino acid , materials science , nanoparticle , nanotechnology , inorganic chemistry , organic chemistry , biochemistry , mathematical analysis , mathematics , optoelectronics
The apoferritin protein and apoferritin–Tb 3+ complex were demonstrated to form oligomeric and polymeric self‐assemblies in neutral aqueous solutions, based on characterization by using luminescence and UV/Vis spectroscopy, dynamic light scattering, and transmission electron microscopy. Addition of a 20‐mer or higher poly(arginine) to the solution resulted in coprecipitation through nanoscale interactions, while biological proteins and other poly(amino acids) rarely yielded precipitates under the conditions employed. The apoferritin–Tb 3+ complex assembly exhibited a particularly long‐lived green luminescence in aqueous solution, and its poly(arginine)‐selective precipitation behavior was followed by monitoring the changes in luminescence. The poly(arginine)‐tagged albumin precipitated selectively and quantitatively, so that the apoferritin–Tb 3+ complex can function as a new luminescent biotool for the sensing of poly(arginine) and its protein conjugates.