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A Non‐Enzymatic, DNA Template‐Directed Morpholino Primer Extension Approach
Author(s) -
Bell Neil M.,
Wong Raymond,
Micklefield Jason
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200902237
Subject(s) - primer extension , extension (predicate logic) , primer (cosmetics) , morpholino , computer science , dna , computational biology , chemistry , combinatorial chemistry , information retrieval , biochemistry , biology , programming language , base sequence , gene , organic chemistry , zebrafish
Extension, please! A non‐enzymatic DNA‐template‐directed synthesis approach has been developed that allows the morpholino‐nucleoside extension of a PNA primer. Equilibration of the ribonucleosides with the template–primer complex, in a non‐covalent fashion (see scheme), was shown to be essential for high selectivity. It is envisaged that this chemistry might be used to transcribe nature's genetic material into alternative abiological polymers.