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Durable Mesenchymal Stem Cell Labelling by Using Polyhedral Superparamagnetic Iron Oxide Nanoparticles
Author(s) -
Wang HaoHao,
Wang YiXiang J.,
Leung Ken ChamFai,
Au Doris W. T.,
Xuan Shouhu,
Chak ChunPong,
Lee Simon K. M.,
Sheng Hui,
Zhang Ge,
Qin Ling,
Griffith James F.,
Ahuja Anil T.
Publication year - 2009
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200901548
Subject(s) - mesenchymal stem cell , labelling , nanoparticle , superparamagnetism , chemistry , chondrogenesis , in vitro , nanotechnology , materials science , biochemistry , microbiology and biotechnology , physics , magnetization , quantum mechanics , magnetic field , biology
Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO 2 and SPIO@SiO 2 ‐NH 2 ). Surface modification of the small polyhedral silica‐coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface‐modified nanoparticles (SPIO@ SiO 2 ‐NH 2 ) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling of SPIO@SiO 2 and SPIO@SiO 2 ‐NH 2 did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO 2 ‐NH 2 nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO 2 ‐NH 2 nanoparticle‐labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long‐lasting, durable MRI labelling efficacy after 8–12 weeks.