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Designing Caspase‐3 Sensors for Imaging of Apoptosis in Living Cells
Author(s) -
Chen Ning,
Huang Yun,
Yang Lily,
Liu Rihe,
Yang Jenny J.
Publication year - 2009
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200901439
Subject(s) - green fluorescent protein , apoptosis , caspase , intracellular , microbiology and biotechnology , caspase 3 , fluorescence , chemistry , sensitivity (control systems) , biophysics , biochemistry , biology , programmed cell death , gene , physics , engineering , electronic engineering , optics
Powerful ratiometric sensor : The caspase–cascade system plays a vital role in intracellular apoptotic signals associated with various human diseases. A single EGFP‐based (EGFP, enhanced green fluorescent protein) caspase‐3 sensor was successfully developed to track the activation and inhibition of caspase‐3 in living cells in real time with a high sensitivity and enzymatic selectivity in a ratiometric measurement mode.