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Use of an Osmium Complex as a Universal Luminescent Probe for Enzymatic Reactions
Author(s) -
Virel Ana,
SanchezLopez Jose,
Saa Laura,
García Ana Carla,
Pavlov Valeri
Publication year - 2009
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200900498
Subject(s) - osmium , chemistry , aqueous solution , fluorescence , redox , yield (engineering) , pyridine , hydrogen peroxide , photochemistry , quantum yield , inorganic chemistry , combinatorial chemistry , organic chemistry , catalysis , ruthenium , physics , materials science , quantum mechanics , metallurgy
Enzymatic modulation of fluorescence : The oxidised form of the Os III complex is fluorescent in aqueous solution, whereas the reduced Os II form shows no significant fluorescence. This universal approach can be easily adapted to follow any enzymatic reaction to yield oxidising or reducing products capable of interacting with the reduced or oxidised form of the osmium complex, respectively (see figure).The water‐soluble bis(bipyridine)chloro(4‐picolinic acid) osmium complex, [Os III (bpy) 2 Cl(PyCOOH)] 2+ (bpy=2,2’‐bipyridine, Py=pyridine), is fluorescent in aqueous solution, whereas the reduced form of the complex, [Os II (bpy) 2 Cl(PyCOOH)] + , shows no significant fluorescence under the same conditions. Such reversible redox control of the fluorescence of the complex can be easily adapted to follow any enzymatic reaction to yield oxidising or reducing products that are capable of interacting with [Os III (bpy) 2 Cl(PyCOOH)] 2+ or [Os II (bpy) 2 Cl(PyCOOH)] + . Based on the redox reaction between products of biocatalytic reactions and the osmium complex, we have designed a simple bioanalytical assay for the detection of nerve gases, α‐ketoglutarate, hydrogen peroxide and glucose.