Premium
2,6,8‐Trisubstituted 3‐Hydroxychromone Derivatives as Fluorophores for Live‐Cell Imaging
Author(s) -
Dyrager Christine,
Friberg Annika,
Dahlén Kristian,
FridénSaxin Maria,
Börjesson Karl,
Wilhelmsson L. Marcus,
Smedh Maria,
Grøtli Morten,
Luthman Kristina
Publication year - 2009
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200900279
Subject(s) - fluorescence , hela , chemistry , photochemistry , fluorescence lifetime imaging microscopy , combinatorial chemistry , biophysics , cell , biochemistry , optics , physics , biology
We present the synthesis and photophysical characterisation of a series of structurally diverse, fluorescent 2,6,8‐trisubstituted 3‐hydroxychromone derivatives with high fluorescence quantum yields and molar extinction coefficients. Two of these derivatives ( 9 and 10 a ) have been studied as fluorophores for cellular imaging in HeLa cells and show excellent permeability and promising fluorescence properties in a cellular environment. In addition, we have demonstrated by photophysical characterisation of 3‐isobutyroxychromone derivatives that esterification of the 3‐hydroxyl group results in acceptable and useful fluorescence properties.