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Determination of Enantiomeric Compositions by Transient Absorption Spectroscopy using Proteins as Chiral Selectors
Author(s) -
Vayá Ignacio,
Bueno Carlos J.,
Jiménez M. Consuelo,
Miranda Miguel A.
Publication year - 2008
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200801657
Subject(s) - enantiomer , transient (computer programming) , chemistry , absorption (acoustics) , trace (psycholinguistics) , spectroscopy , composition (language) , computer science , organic chemistry , physics , optics , linguistics , philosophy , quantum mechanics , operating system
Which enantiomer? A new method for determination of enantiomeric compositions is presented here. It is based on transient absorption spectroscopy, exploiting the stereodifferentiation of triplet lifetimes within serum albumins (SA) for chiral recognition (see scheme). Multiexponential fitting of the triplet decay trace leads to the preexponential factors corresponding to the contribution of the S and R enantiomers within the protein binding sites I and II.