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Nano‐Conjugate Fluorescence Probe for the Discrimination of Phosphate and Pyrophosphate
Author(s) -
Kim IkBum,
Han Man H.,
Phillips Ronnie L.,
Samanta Bappaditya,
Rotello Vincent M.,
Zhang Z. John,
Bunz Uwe H. F.
Publication year - 2009
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200801403
Subject(s) - pyrophosphate , conjugate , fluorescence , nano , phosphate , chemistry , nanotechnology , materials science , biochemistry , optics , physics , mathematics , composite material , mathematical analysis , enzyme
We describe a pyrophosphate (PPi) probe that is based on a fluorescent dicarboxylate‐substituted poly( para‐ phenyleneethynylene) (PPE) and 10 nm cobalt–iron spinel nanoparticles (NPs) in aqueous media. The spinel NPs efficiently quench the fluorescence of the PPE at a concentration of 20–30 pmol. Addition of phosphate anions to the PPE–NP construct displaces the quenched PPE to give rise to a fluorescent response; we found that PPi and phosphate (Pi) have significantly different binding affinities for the self‐assembled materials. We can discern >40 n M PPi in the presence of 0.1 m M Pi at pH 7, which suggests that these assemblies may be useful in bio‐analytical applications. This displacement assay was used to effectively determine the ability of pyrophosphatase to hydrolyze PPi to Pi.