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Intracellular Uptake and Photodynamic Activity of Water‐Soluble [60]‐ and [70]Fullerenes Incorporated in Liposomes
Author(s) -
Doi Yuki,
Ikeda Atsushi,
Akiyama Motofusa,
Nagano Mai,
Shigematsu Tamami,
Ogawa Takuya,
Takeya Tatsuo,
Nagasaki Takeshi
Publication year - 2008
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200801090
Subject(s) - phototoxicity , singlet oxygen , fullerene , photodynamic therapy , hela , chemistry , photochemistry , liposome , biophysics , absorption (acoustics) , fluorescence , vesicle , nanotechnology , membrane , oxygen , materials science , biochemistry , organic chemistry , cell , biology , physics , in vitro , quantum mechanics , composite material
Water‐soluble fullerenes have attracted attention as promising compounds that have been used to forge new paths in the field of photo‐biochemistry. To prepare water‐soluble fullerenes, we employed lipid‐membrane‐incorporated fullerenes (LMIC x ; x =60 or 70) by using the fullerene exchange method from a γ‐cyclodextrin (γ‐CD) cavity to vesicles. LMIC 60 have low toxicity in the dark and engender cell death by photoirradiation ( λ >350 nm). Furthermore, the photodynamic activity of LMIC 70 is 4.7‐fold that of LMIC 60 for the same photon flux ( λ >400 nm). One of the reasons for the higher phototoxicity of LMIC 70 is the higher generation of singlet oxygen ( 1 O 2 ) in LMIC 70 than in LMIC 60 . The difference between LMIC 60 and LMIC 70 is considered to be simply derived from the amount of light absorption in the 400–700 nm region that is suitable for photodynamic therapy (PDT). To the best of our knowledge, this is the first case in which biological activity of C 70 and its derivatives toward HeLa cells has been assayed.