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Highly Sensitive and Fast Responsive Fluorescence Turn‐On Chemodosimeter for Cu 2+ and Its Application in Live Cell Imaging
Author(s) -
Yu Mengxiao,
Shi Mei,
Chen Zhigang,
Li Fuyou,
Li Xinxin,
Gao Yanhong,
Xu Jia,
Yang Hong,
Zhou Zhiguo,
Yi Tao,
Huang Chunhui
Publication year - 2008
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200800005
Subject(s) - chemistry , fluorescence , metal ions in aqueous solution , photochemistry , live cell imaging , rhodamine , copper , quenching (fluorescence) , metal , absorption (acoustics) , fluorescence in the life sciences , ion , förster resonance energy transfer , materials science , biochemistry , physics , organic chemistry , quantum mechanics , composite material , cell
A rhodamine B derivative 4 containing a highly electron‐rich S atom has been synthesized as a fluorescence turn‐on chemodosimeter for Cu 2+ . Following Cu 2+ ‐promoted ring‐opening, redox and hydrolysis reactions, comparable amplifications of absorption and fluorescence signals were observed upon addition of Cu 2+ ; this suggests that chemodosimeter 4 effectively avoided the fluorescence quenching caused by the paramagnetic nature of Cu 2+ . Importantly, 4 can selectively recognize Cu 2+ in aqueous media in the presence of other trace metal ions in organisms (such as Fe 3+ , Fe 2+ , Cu + , Zn 2+ , Cr 3+ , Mn 2+ , Co 2+ , and Ni 2+ ), abundant cellular cations (such as Na + , K + , Mg 2+ , and Ca 2+ ), and the prevalent toxic metal ions in the environment (such as Pb 2+ and Cd 2+ ) with high sensitivity (detection limit ≤10 ppb) and a rapid response time (≤1 min). Moreover, by virtue of the chemodosimeter as fluorescent probe for Cu 2+ , confocal and two‐photon microscopy experiments revealed a significant increase of intracellular Cu 2+ concentration and the subcellular distribution of Cu 2+ , which was internalized into the living HeLa cells upon incubation in growth medium supplemented with 50 μ M CuCl 2 for 20 h.