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Theoretical Investigation of the Reaction Mechanism of the Dinuclear Zinc Enzyme Dihydroorotase
Author(s) -
Liao RongZhen,
Yu JianGuo,
Raushel Frank M.,
Himo Fahmi
Publication year - 2008
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200701948
Subject(s) - chemistry , nucleophile , protonation , tetrahedral carbonyl addition compound , amide , hydroxide , active site , density functional theory , zinc , reaction mechanism , stereochemistry , hydrogen bond , kinetic isotope effect , photochemistry , enzyme , computational chemistry , inorganic chemistry , molecule , deuterium , organic chemistry , catalysis , ion , physics , quantum mechanics
The reaction mechanism of the dinuclear zinc enzyme dihydroorotase was investigated by using hybrid density functional theory. This enzyme catalyzes the reversible interconversion of dihydroorotate and carbamoyl aspartate. Two reaction mechanisms in which the important active site residue Asp250 was either protonated or unprotonated were considered. The calculations establish that Asp250 must be unprotonated for the reaction to take place. The bridging hydroxide is shown to be capable of performing nucleophilic attack on the substrate from its bridging position and the role of Zn β is argued to be the stabilization of the tetrahedral intermediate and the transition state leading to it, thereby lowering the barrier for the nucleophilic attack. It is furthermore concluded that the rate‐limiting step is the protonation of the amide nitrogen by Asp250 coupled with CN bond cleavage, which is consistent with previous experimental findings from isotope labeling studies.