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Ionic Liquids as Selectors for the Enhanced Detection of Proteins
Author(s) -
Li Tao,
Li Bingling,
Dong Shaojun,
Wang Erkang
Publication year - 2007
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200700275
Subject(s) - capillary electrophoresis , chromatography , ionic liquid , chemistry , avidin , capillary action , buffer (optical fiber) , electrochemiluminescence , ionic strength , electrophoresis , adsorption , analytical chemistry (journal) , materials science , detection limit , biotinylation , computer science , biochemistry , telecommunications , organic chemistry , aqueous solution , composite material , catalysis
Herein, we report an approach for protein detection enhanced by ionic liquid (IL) selectors in capillary electrophoresis (CE), with avidin as a model protein. Hydrophilic ILs were added into the running buffer of CE and acted as selectors for sample injection, enriching the positive target and excluding the negative from the capillary. When using 3 % (v/v) IL selector, the detection sensitivity of avidin was improved by over one order of magnitude, while the interference from protein adsorption was effectively avoided, even in an uncoated capillary. The electrochemiluminescence method was initially used for IL‐based CE with low noise that was independent of the IL concentration, making ILs almost transparent as additives in the electrophoresis buffer.