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Formation and Stability of G‐Quadruplexes Self‐Assembled from Guanine‐Rich Strands
Author(s) -
Zhou Jiang,
Yuan Gu,
Liu Junjun,
Zhan ChangGuo
Publication year - 2007
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200600424
Subject(s) - g quadruplex , guanine , chemistry , biophysics , dna , biochemistry , nucleotide , biology , gene
Abstract Electrospray ionization mass spectrometry (ESI‐MS) was utilized to investigate the formation and stability of G‐quadruplexes. For the 15 6‐nt oligonucleotides tested, ESI‐MS indicated that formation of a parallel tetramer quadruplex requires at least four continuous guanines in the 6‐nt sequence. In addition, the G‐rich strands prefer to employ “self‐association” in the formation of the G‐quadruplex rather than hybridized integration, and the thermodynamic‐stability order of these three G‐quadruplexes is Q(2)>Q(1)>Q(3).