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Affinity Adhesion of Carbohydrate Particles and Yeast Cells to Boronate‐Containing Polymer Brushes Grafted onto Siliceous Supports
Author(s) -
Ivanov Alexander E.,
Panahi Homayon Ahmad,
Kuzimenkova Marina V.,
Nilsson Lars,
Bergenståhl Björn,
Waqif Husain S.,
Jahanshahi Mohsen,
Galaev Igor Yu.,
Mattiasson Bo
Publication year - 2006
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200600342
Subject(s) - agarose , monolayer , chemistry , adhesion , copolymer , adsorption , polymer , yeast , polymer chemistry , sepharose , protein adsorption , chemical engineering , biophysics , chromatography , biochemistry , organic chemistry , enzyme , biology , engineering
Cross‐linked agarose particles (Sepharose CL‐6B) and baker's yeast cells were found to adhere to siliceous supports end‐grafted with boronate‐containing copolymers (BCCs) of N , N ‐dimethylacrylamide at pH≥7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH≥7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE‐3B formed stable, monolayer‐like structures at pH 8.0, whereas at pH 7.0–7.8 the structures on the copolymer‐grafted supports were less stable and more random. At pH 9.0, 50 % saturation of the surface with adhering cells was attained in 2 min. Stained cells formed denser and more stable layers on the copolymer‐grafted supports than they did on supports modified with self‐assembled organosilane layers derivatized with low‐molecular‐weight boronate, presumably due to a higher reactivity of the grafted BCCs. Quantitative detachment of adhered particles and cells could be achieved by addition of 20 m M fructose—a strong competitor for binding to boronates—at pH 7.0–9.0. Regeneration of the grafted supports allowed several sequential adhesion and detachment cycles with stained yeast cells. Affinity adhesion of micron‐sized carbohydrate particles to boronate‐containing polymer brushes fixed on solid supports is discussed as a possible model system suggesting a new approach to isolation and separation of living cells.