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Cover Picture: Intramolecular Dimers: A New Design Strategy for Fluorescence‐Quenched Probes (Chem. Eur. J. 15/2003)
Author(s) -
Johansson Mary Katherine,
Cook Ronald M.
Publication year - 2003
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200390237
Subject(s) - intramolecular force , fluorescence , quenching (fluorescence) , chemistry , dimer , oligonucleotide , photochemistry , cover (algebra) , stereochemistry , optics , physics , mechanical engineering , engineering , dna , biochemistry , organic chemistry
The cover picture shows a 96‐well plate illuminated by a UV lamp and viewed through a cutoff filter. All wells contain a Cy3.5‐BHQ1 (reporter quencher) dual‐labeled oligonucleotide probe in buffer. Complementary sequence was added to three columns. The probe is virtually nonfluorescent due to static quenching through formation of an intramolecular dimer. Addition of complement separates the dye and quencher, causing fluorescence. Intramolecular dimers as a new design strategy for fluorescence‐quenched probes is discussed by M. K. Johansson and R. M. Cook in the Concepts article on p. 3466 ff.