Premium
Superbeads: Immobilization in “Sweet” Chemistry
Author(s) -
Nahalka Jozef,
Liu Ziye,
Chen Xi,
Wang Peng George
Publication year - 2003
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200390038
Subject(s) - glycosylation , chemistry , glycosyltransferase , recombinant dna , nucleotide sugar , oligosaccharide , enzyme , sugar , biochemistry , combinatorial chemistry , gene
Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.