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Voltage‐Dependent Formation of Anion Channels by Synthetic Rigid‐Rod Push–Pull β ‐Barrels
Author(s) -
Sakai Naomi,
Houdebert David,
Matile Stefan
Publication year - 2003
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200390016
Subject(s) - chemistry , bilayer , conductance , ion , quenching (fluorescence) , ion channel , chemical physics , fluorescence , crystallography , vesicle , lipid bilayer , circular dichroism , membrane , optics , organic chemistry , biochemistry , physics , receptor , mathematics , combinatorics
Ion channels formed by p ‐octiphenyls equipped with amphiphilic, cationic tripeptide strands and either with ( 5 ) or without ( 6 ) axial dipole moment are described (preliminary communication: N. Sakai, S. Matile, J. Am. Chem. Soc. 2002 , 124 , 1184–1185). Fluorescence kinetics with variably polarized neutral or anionic vesicles, together with planar bilayer conductance measurements, reveal voltage dependence with weakly lyotropic anion selectivity, and deactivation by competing surface potentials of the ion channels formed by asymmetric 5 . In planar bilayers, 5 forms short‐lived, poorly organized channels—similar to those produced by α ‐helical natural antibiotics—capable of transforming into stable, ohmic p ‐octiphenyl “ β ‐barrel” ion channels similar to those of the >99 % homologous but symmetric 6 . Fluorescence depth quenching and circular dichroism studies confirm the effect of membrane potentials in promotion of the partitioning of 5 (but not 6 ) into the bilayers, identifying partitioning as the voltage‐dependent step.