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Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins
Author(s) -
Siegers Conrad,
Biesalski Markus,
Haag Rainer
Publication year - 2004
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200306073
Subject(s) - monolayer , ethylene glycol , protein adsorption , polymer , contact angle , adsorption , self assembled monolayer , materials science , surface plasmon resonance , chemical engineering , surface modification , peg ratio , linker , polymer chemistry , chemistry , thermal stability , combinatorial chemistry , organic chemistry , nanotechnology , nanoparticle , composite material , finance , computer science , engineering , economics , operating system
Highly protein‐resistant, self‐assembled monolayers (SAMs) of dendritic polyglycerols (PGs) on gold can easily be obtained by simple chemical modification of these readily available polymers with a surface‐active disulfide linker group. Several disulfide‐functionalized PGs were synthesized by N , N ′‐dicyclohexylcarbodiimide‐mediated ester coupling of thioctic acid. Monolayers of the disulfide‐functionalized PG derivatives spontaneously form on a semitransparent gold surface and effectively prevent the adsorption of proteins, as demonstrated by surface plasmon resonance (SPR) kinetic measurements. A structure–activity relationship relating the polymer architecture to its ability to effectuate protein resistance has been derived from results of different surface characterization techniques (SPR, attenuated total reflectance infrared (ATR‐IR), and contact‐angle measurements). Dendritic PGs combine the characteristic structural features of several highly protein‐resistant surfaces: a highly flexible aliphatic polyether, hydrophilic surface groups, and a highly branched architecture. PG monolayers are as protein resistant as poly(ethylene glycol) (PEG) SAMs and are significantly better than dextran‐coated surfaces, which are currently used as the background for SPR spectroscopy. Due to the higher thermal and oxidative stability of the bulk PG as compared to the PEG and the easy accessibility of these materials, dendritic polyglycerols are novel and promising candidates as surface coatings for biomedical applications.