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Au Nanoparticle‐Enhanced Surface Plasmon Resonance Sensing of Biocatalytic Transformations
Author(s) -
Zayats Maya,
Pogorelova Svetlana P.,
Kharitonov Andrei B.,
Lioubashevski Oleg,
Katz Eugenii,
Willner Itamar
Publication year - 2003
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200305104
Subject(s) - surface plasmon resonance , nanoparticle , nanotechnology , materials science , plasmon , resonance (particle physics) , surface plasmon , localized surface plasmon , chemistry , optoelectronics , physics , particle physics
N ‐(3‐Aminopropyl)‐ N′ ‐methyl‐4,4′‐bipyridinium is coupled to tiopronin‐capped Au nanoparticles (diameter ca. 2 nm) to yield methyl(aminopropyl)viologen‐functionalized Au nanoparticles (MPAV 2+ ‐Au nanoparticles). In situ electrochemical surface plasmon resonance (SPR) measurements are used to follow the electrochemical deposition of the bipyridinium radical cation modified Au nanoparticles on an Au‐coated glass surface and the reoxidation and dissolution of the bipyridinium radical cation film. The MPAV 2+ ‐functionalized Au nanoparticles are also employed for the amplified SPR detection of NAD + and NADH cofactors. By SPR monitoring the partial biocatalyzed dissolution of the bipyridinium radical cation film in the presence of diaphorase (DP) NAD + is detected in the concentration range of 1×10 −4 M to 2×10 −3 M . Similarly, the diaphorase‐mediated formation of the bipyridinium radical cation film on the Au‐coated glass surface by the reduction of the MPAV 2+ ‐functionalized Au nanoparticles by NADH is used for the amplified SPR detection of NADH in the concentration range of 1×10 −4 M to 1×10 −3 M .