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Discrimination of Peptides by Using a Molecularly Imprinted Piezoelectric Biosensor
Author(s) -
Lin ChungYin,
Tai DarFu,
Wu TzongZeng
Publication year - 2003
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200304952
Subject(s) - quartz crystal microbalance , molecularly imprinted polymer , biosensor , monomer , molecular imprinting , polymer , polymerization , molecular recognition , chemistry , peptide , combinatorial chemistry , selectivity , chromatography , aqueous solution , materials science , nanotechnology , adsorption , molecule , organic chemistry , biochemistry , catalysis
Based on the direct formation of a molecularly imprinted polymer on gold electrodes, we have developed a peptide sensor for the detection of low‐molecular‐weight peptides. A new cross‐linking monomer, ( N ‐Acr‐ L ‐Cys‐NHBn) 2 , was employed to attach the surface of the chip and to copolymerize with other monomers. Interestingly, N ‐benzylacrylamide participates in the polymerization and recognition is carried out in an aqueous environment. By using quartz crystal microbalance detection, short peptides can be monitored by their interaction with plastic antibodies specific for the target peptides. The selectivity of molecularly imprinted polymers and the sensitivity of such artificial biosensors have been combined to differentiate between traces of oxytocin and vasopressin to the ng mL −1 scale.