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Optimization of Hydrolase Efficiency in Organic Solvents
Author(s) -
Secundo Francesco,
Carrea Giacomo
Publication year - 2003
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200304813
Subject(s) - biochemical engineering , hydrolase , aqueous medium , organic synthesis , chemistry , catalysis , catalytic efficiency , organic solvent , enzyme catalysis , organic chemistry , combinatorial chemistry , computer science , aqueous solution , process engineering , enzyme , chemical engineering , engineering
The application of hydrolases in organic solvents for synthetic purposes is a procedure routinely adopted in organic chemistry, especially for the preparation of chiral building blocks. Numerous studies have shed light on several aspects of the mechanism of hydrolase action in low‐water environments. Procedures suitable to improve the catalytic efficiency of enzymes and productivity of the synthetic processes have been reported. These fundamental and applied investigations have made hydrolase‐catalyzed reactions in organic solvents of industrial interest. In this article we describe and discuss various approaches adopted to optimize the performance of hydrolases in organic media, with special emphasis on the formulation of the biocatalysts which, under proper conditions, can display an activity equal to that displayed in aqueous buffers.