Premium
1′,5′‐Anhydrohexitol Oligonucleotides: Hybridisation and Strand Displacement with Oligoribonucleotides, Interaction with RNase H and HIV Reverse Transcriptase
Author(s) -
Hendrix Chris,
Rosemeyer Helmut,
De Bouvere Bart,
Van Aerschot Arthur,
Seela Frank,
Herdewijn Piet
Publication year - 1997
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.19970030920
Subject(s) - rnase h , reverse transcriptase , rna , oligonucleotide , rnase p , duplex (building) , dna , nucleic acid , coding strand , microbiology and biotechnology , nuclease protection assay , cleavage (geology) , chemistry , biology , biochemistry , rna dependent rna polymerase , gene , paleontology , fracture (geology)
Abstract Hexitol nucleic acids (HNAs) with four natural bases form stable and sequence‐selective duplexes with RNA. This was investigated by T m determinations and gel shift experiments. The CD spectra of an HNA‐RNA duplex show similarities with the CD spectra of the A‐form of dsRNA. Single‐stranded HNAs are able to induce strand displacement in a double‐stranded RNA sequence. An HNA‐RNA duplex is a poor substrate for RNase H, and can inhibit the RNase H‐mediated cleavage of a natural DNA‐RNA substrate. The HNA‐RNA hybrid enhances the activity of HIV reverse transcriptase.