Premium
Regioselective Deuteration and Resonance Raman Spectroscopic Characterization of Biliverdin and Phycocyanobilin
Author(s) -
Knipp Bernd,
Kneip Krista,
Matysik Jörg,
Gärtner Wolfgang,
Hildebrandt Peter,
Braslavsky Silvia E.,
Schaffner Kurt
Publication year - 1997
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.19970030307
Subject(s) - biliverdin , phycocyanobilin , raman spectroscopy , resonance (particle physics) , chemistry , regioselectivity , resonance raman spectroscopy , allene , nuclear magnetic resonance , stereochemistry , heme , organic chemistry , physics , biology , catalysis , atomic physics , heme oxygenase , enzyme , optics , cyanobacteria , phycocyanin , bacteria , genetics
5‐Deuteriophycocyanobilin ( 4 ), 5,15,18 2 ,18 2 ‐tetradeuteriobiliverdin IXα dimethyl ester ( 6 ), and 5,15‐dideuteriomesobiliverdin IXα dimethyl ester ( 8 ) have been prepared and analyzed by resonance Raman spectroscopy in the C–H out‐of‐plane region, based on a previous normal‐mode analysis of biliverdin XI α dimethyl ester ( 5 ). In this way most of the modes containing out‐of‐plane vibrations of the methine bridges could be assigned unambiguously. These results are of special relevance with regard to the interpretation of the resonance Raman spectra of the chromoprotein phytochrome.