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A mathematical model of measurement uncertainty of single substrate enzyme assays
Author(s) -
Ramamohan Varun,
Abbott James T.,
Yih Yuehwern
Publication year - 2015
Publication title -
journal of chemometrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.47
H-Index - 92
eISSN - 1099-128X
pISSN - 0886-9383
DOI - 10.1002/cem.2661
Subject(s) - measurement uncertainty , process (computing) , uncertainty analysis , computer science , monte carlo method , observational error , lactate dehydrogenase , calibration , experimental data , uncertainty quantification , biological system , data mining , statistics , mathematics , chemistry , enzyme , simulation , machine learning , biology , biochemistry , operating system
Clinical laboratory tests provide critical information at every stage of the medical decision‐making process, and measurement of the activity levels of enzymes such as alkaline phosphatase, lactate dehydrogenase, etc. provide information regarding various body functions such as the liver and gastrointestinal tract. The uncertainty associated with these enzyme measurement processes describes the quality of the measurement process, and therefore methods to improve the quality of the measurement process require minimizing the measurement uncertainty of the enzyme assay. In this study, we develop a mathematical model of the lactate dehydrogenase measurement process, with uncertainty introduced into its parameters that represent the sources of variation in the different components and stages of the measurement process. The Monte Carlo method is then utilized to estimate the uncertainty associated with the model, and therefore the measurement process. An empirical function used to generate estimates of uncertainty for patient samples with unknown activity levels is constructed using the model. The model is then used to quantify the contributions of the individual sources of uncertainty to the net measurement uncertainty and also quantify the effect of uncertainty within the calibration process on the distribution of the measurement result. Copyright © 2014 John Wiley & Sons, Ltd.