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Rapid estimation of the number of coeluting components in liquid chromatography by factor analysis
Author(s) -
Shen Chengbo,
Vickers Thomas J.,
Mann Charles K.
Publication year - 1991
Publication title -
journal of chemometrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.47
H-Index - 92
eISSN - 1099-128X
pISSN - 0886-9383
DOI - 10.1002/cem.1180050503
Subject(s) - plot (graphics) , chromatography , chemistry , function (biology) , spectral line , spectral density , biological system , analytical chemistry (journal) , mathematics , statistics , physics , astronomy , evolutionary biology , biology
Abstract Evaluation of the results of factor analysis of sets of spectroscopically detected chromatograms is carried out by examining the shapes of the abstract factors. This is done either by visual inspection or by analysis of the power density spectra produced from them. Owing to constraints imposed by the column function and the spectroscopic instrument function, the information content of the chromatograms necessarily occurs at low spatial frequencies. As a consequence, it appears as relatively broad features in the abstract chromatograms and as a peak in the low‐frequency region of the corresponding power density plot. On the basis of examination of the power density distribution, a well‐defined distinction is made between primary and secondary abstract factors. The major uncertainty encountered in determining the number of chemical components appears to arise from effects of contaminants in reagents.