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Evaluation of curve resolution and iterative target transformation factor analysis in quantitative analysis by liquid chromatography
Author(s) -
Vandeginste B. G. M.,
Leyten F.,
Gerritsen M.,
Noor J. W.,
Kateman G.,
Frank J.
Publication year - 1987
Publication title -
journal of chemometrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.47
H-Index - 92
eISSN - 1099-128X
pISSN - 0886-9383
DOI - 10.1002/cem.1180010108
Subject(s) - chromatography , resolution (logic) , chemistry , quantitative analysis (chemistry) , analytical chemistry (journal) , high performance liquid chromatography , transformation (genetics) , elution , reversed phase chromatography , chromatography detector , computer science , artificial intelligence , biochemistry , gene
The performance of curve resolution and iterative target transformation curve resolution in quantitative liquid chromatography with a diode array detector is evaluated. Quantitative accuracy of the elution profiles obtained for two‐ and three‐component mixtures has been evaluated as a function of spectral similarity, chromatographic resolution and ratio of the peak areas, using a number of simulations analysed by an analysis of variance. The results obtained by simulation are compared and validated with the results obtained for the separation of phenylene diamines in reversed phase liquid chromatography and proteins in gel permeation liquid chromatography. The results that were obtained are summarized and the chromatographic implications are discussed.