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Trace miRNA Assay Based on DNA Nanostructures Formed by Hybridization Chain Reaction and Gold‐Nanoparticle Tags
Author(s) -
Song Yan,
Wang Mingyuan,
Qian Qin,
Xu Jun,
Zhou Qungang,
Lv Shujie,
Miao Peng
Publication year - 2021
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.202100466
Subject(s) - colloidal gold , microrna , nanotechnology , biosensor , dna , nanoparticle , chain reaction , electrode , nanostructure , computational biology , electrochemistry , oligonucleotide , chemistry , materials science , combinatorial chemistry , biology , gene , biochemistry , photochemistry
microRNAs (miRNAs), a class of endogenous non‐coding RNAs, are involved in many crucial biological processes, which have emerged as a new set of biomarkers for disease theranostics. Exploring efficient signal amplification strategy is highly desired to pursue an ultrasensitive miRNA biosensing platform. In this work, we have developed a novel electrochemical approach for the analysis of trace miRNA. Target triggered DNA assembly is performed on the surface of the electrode. Meanwhile, gold‐nanoparticle tags are integrated on the product of hybridization chain reaction, which further enhance electrochemical response towards initiator miRNA. The as‐prepared electrochemical biosensor shows good analytical performances and satisfactory practical utility. It has great potential to provide a powerful tool for the miRNA assay in biomedical fields.