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Spatially Resolved Electron Transport through Anode‐Respiring Geobacter sulfurreducens Biofilms: Controls and Constraints
Author(s) -
He Xiaojia,
Chadwick Grayson,
Jiménez Otero Fernanda,
Orphan Victoria,
Meile Christof
Publication year - 2021
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.202100111
Subject(s) - geobacter sulfurreducens , microbial fuel cell , biofilm , geobacter , electron transfer , anode , electron transport chain , biophysics , chemistry , cathode , redox , biological system , chemical physics , biology , bacteria , electrode , biochemistry , inorganic chemistry , photochemistry , genetics
Microbial fuel cells (MFCs) with Geobacter sulfurreducens have been shown to produce high current densities; however, electron transport in G. sulfurreducens biofilms is not fully understood. Here, we utilize a spatially resolved numerical model describing this electron transfer to constrain mechanisms and controls on metabolic activity. Our model reproduces the metabolic activity profile obtained using nanoSIMS under positive (+0.24 V SHE) and negative (−0.1 V SHE) anode potentials. The simulations indicate that the distribution of the electric potential and pH both control cellular metabolism. Model simulations reproducing the experimentally determined activity patterns also support the presence of two activity modes in G. sulfurreducens biofilms, with a shift from a redox mid‐potential of −0.07 V SHE to −0.15 V SHE. Our model provides valuable insights into the fundamental mechanisms of electron transfer at Micron‐scale in conductive biofilms which can inform MFCs designs that maximize current production by minimizing the impact of inhibitory factors.