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Surface Patterning of Biomolecules Using Click Chemistry and Light‐Activated Electrochemistry to Locally Generate Cu(I)
Author(s) -
Gautam Shreedhar,
Lian Jiaxin,
R. Gonçales Vinicius,
Vogel Yan B.,
Ciampi Simone,
Tilley Richard D.,
Gooding J. Justin
Publication year - 2020
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.202001097
Subject(s) - click chemistry , biomolecule , ethylene glycol , electrode , nanotechnology , molecule , electrochemistry , adhesion , chemistry , alkyne , covalent bond , silicon , materials science , catalysis , optoelectronics , polymer chemistry , organic chemistry
Here we report a light‐assisted variant of click chemistry suitable for chemical patterning of semiconducting surfaces. Using a visible light beam to illuminate desired locations of an electrode surface, localized electrogeneration of the Cu(I) click catalyst was achieved. It was demonstrated that surface click reactions were correlated with a user‐defined 2D light pattern projected on a silicon photoelectrode. This new process is mask‐free and parallel, i. e., separate discrete regions of a semiconducting surface can be simultaneously reacted with a range of functional molecules. The covalent patterning of azido‐poly (ethylene glycol) molecules (PEG) over alkyne‐functionalized electrodes was employed as a proof‐of‐principle. By modulating the exposed chemical group of the clicked azido‐PEGs, the adhesion of antibodies and cells was further possible with spatial control. The use of structured light, instead of physical masks, drastically reduces patterning time to a couple of hours.