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Photoelectrochemical Immunosensor for Sensitive Quantification of Prostate Specific Antigen in Human Serum Samples Exploiting BaTiO 3 −CdS
Author(s) -
Reis Lima Fernanda M.,
Soares RossyEric P.,
Sinfrônio Francisco Sávio M.,
Maciel Adeilton P.,
Menezes Alan S.,
Pereira Silma Regina F.,
Damos Flavio S.,
Luz Rita de Cássia S.
Publication year - 2020
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.202000801
Subject(s) - photocurrent , tin oxide , chemistry , detection limit , materials science , cadmium sulfide , chromatography , doping , inorganic chemistry , optoelectronics
A novel label‐free photoelectrochemical immunosensor was developed for the determination of prostate‐specific antigen (PSA) based on fluorine‐doped tin oxide (FTO) electrode modified with barium titanate and cadmium sulfide (BaTiO 3 −CdS/FTO). The photoelectrochemical current density of the BaTiO 3 platform sensitized with CdS for the ascorbic acid (AA) donor molecule was higher than that observed in the absence of CdS. The BaTiO 3 −CdS/FTO platform was modified with PSA antibodies to produce the anti‐PSA/BaTiO 3 −CdS/FTO immunosensor, which was incubated in solutions of PSA to inhibit the photocurrent density of the immunosensor for AA. The photocurrent density of the anti‐PSA/BaTiO 3 −CdS/FTO immunosensor presented a linear relationship with the logarithm of the PSA concentration ranging from 1 pg mL −1 to 1 μg mL −1 with good sensitivity. The proposed method presented an experimental detection limit of 1 pg mL −1 (S/N=3). The immunosensor was successfully employed for the determination of PSA in blood serum samples, with recoveries between 98.7 %–104.4 %.