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Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework
Author(s) -
Melo Antonio F. A. A.,
Sedenho Graziela C.,
Osica Izabela,
Ariga Katsuhiko,
Crespilho Frank N.
Publication year - 2019
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201901047
Subject(s) - mesoporous material , electrode , materials science , electrochemistry , indium tin oxide , redox , dispersity , nanotechnology , nanoparticle , chemical engineering , colloidal gold , chemistry , catalysis , layer (electronics) , organic chemistry , polymer chemistry , metallurgy , engineering
Protein immobilization on electrode surfaces remains a technological challenge and is decisive in enhancing biological properties with simplified operational steps. In view of proposing a new direction for protein immobilization, we report the development of a mesoporous framework resulting from the magnetically induced assembly of magnetite nanoparticles decorated with near‐monodisperse gold nanoparticles onto an indium‐tin‐oxide electrode. With an electrochemically active surface area of about 72 cm 2 and pores with an average size of 31±1 nm, this magnetically induced mesoporous framework is suitable for the immobilization of small redox proteins. As a proof‐of‐concept, we show the electrochemical process of cytochrome c, where the mesoporous electrode provided a fourfold enhancement for the faradaic currents in comparison to standard polycrystalline gold electrodes.