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Bacillus Licheniformis CotA Laccase Mutant: ElectrocatalyticReduction of O 2 from 0.6 V (SHE) at pH 8 and in Seawater
Author(s) -
Lopes Paula,
Koschorreck Katja,
Nedergaard Pedersen Jannik,
Ferapontov Alexey,
Lörcher Samuel,
Skov Pedersen Jan,
Urlacher Vlada B.,
Ferapontova Elena E.
Publication year - 2019
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201900363
Subject(s) - bacillus licheniformis , seawater , laccase , chemistry , mutant , food science , enzyme , nuclear chemistry , biochemistry , biology , bacillus subtilis , bacteria , ecology , gene , genetics
Enzymes operating in environmental media are of immense biotechnological interest, in particular, for sustainable energy production. Here, we show that the K316 N/D500G mutant of CotA laccase from Bacillus licheniformis operates in basic media and seawater, electrocatalyzing O 2 reduction from 0.59 V, pH 8. CotA mutant's T1Cu center exhibits a formal potential of 0.56 V, consistent with an onset of bioelectrocatalytic reduction of O 2 . A photoelectrochemical cell recycling H 2 O/O 2 by a semiconductor photoanode and CotA mutant biocathode produced 41 and 11 μW cm 2 , in buffer and seawater, respectively, at pH 8, (electricity production being limited solely by the photoanode performance, reaching 240 and 220 μW cm −2 in a Zn‐biobattery design), suggesting a prospective enzyme application for clean and sustainable production of electricity from seawater and oxygen.