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Effect of Electrolyte Ions on the Stability of Flavin Adenine Dinucleotide‐Dependent Glucose Dehydrogenase
Author(s) -
Nemoto Miki,
Sugihara Kazunori,
Adachi Tsubasa,
Murata Kazuki,
Shiraki Kentaro,
Tsujimura Seiya
Publication year - 2019
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201801241
Subject(s) - chemistry , flavin adenine dinucleotide , ammonium sulfate , inorganic chemistry , electrolyte , flavin group , dehydrogenase , sodium , enzyme , nuclear chemistry , biochemistry , chromatography , cofactor , organic chemistry , electrode
The thermal stability of flavin adenine dinucleotide‐dependent glucose dehydrogenase (FAD‐GDH) from Aspergillus terreus in phosphate buffer solution was substantially improved by kosmotropic ions, especially kosmotropic anions. The residual activity of FAD‐GDH in a 1.5 M sodium or ammonium sulfate solution remained more than 90 % after 60 min of heat treatment at 60 °C, while slight activity was observed for FAD‐GDH in a solution in the absence of additive sulfate ions. The stabilizing effect of the electrolytes was concentration‐dependent and strongly related to the structural stabilization of the enzyme, which involved enzyme compaction.