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Flow‐Based Deacidification of Geobacter sulfurreducens Biofilms Depends on Nutrient Conditions: a Microfluidic Bioelectrochemical Study
Author(s) -
Zarabadi Mir Pouyan,
Charette Steve J.,
Greener Jesse
Publication year - 2018
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201800968
Subject(s) - geobacter sulfurreducens , biofilm , geobacter , microfluidics , nutrient , chemistry , environmental chemistry , flow conditions , flow (mathematics) , environmental science , biochemical engineering , bacteria , nanotechnology , biology , materials science , geometry , mathematics , organic chemistry , genetics , engineering
Biofilms from Geobacter sulfurreducens are promising materials for new bioelectrochemical systems. To improve the performance of such systems, limitations related to biofilm acidification should be addressed. This work examines a long‐held assumption that liquid flow can deacidify biofilm pH by enhancing molecular mass transport in the biofilm subdomain. A microfluidic electrochemical system was used to measure changes to biofilm pH in situ while accurately modulating hydrodynamic conditions under turnover, nutrient‐limited and starvation conditions. We discovered that increased flow rates could indeed mitigate biofilm acidification, but not under turnover concentrations, which are the predominant conditions used in research studies. This effect is demonstrated with the observation that relative increases to bio‐current under increased flow rates were stronger for experiments conducted under nutrient‐limited concentrations compared to turnover concentrations. This can open the way for a solution to poor performance of some bioelectrochemical systems at low concentrations.