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Monitoring Potential‐Induced DNA Dehybridization Kinetics for Single Nucleotide Polymorphism Detection by using In Situ Surface Enhanced Raman Scattering
Author(s) -
Kayran Y. Ugur,
Cinar Nergis,
Jambrec Daliborka,
Schuhmann Wolfgang
Publication year - 2018
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201701220
Subject(s) - kinetics , dna , single nucleotide polymorphism , desorption , raman scattering , chemistry , ionic strength , analytical chemistry (journal) , hybridization probe , nucleotide , snp , in situ , biophysics , microbiology and biotechnology , raman spectroscopy , materials science , biochemistry , biology , chromatography , gene , physics , optics , genotype , adsorption , quantum mechanics , aqueous solution , organic chemistry
Changes in temperature, ionic strength or electrical field are generally employed to dehybridize double‐stranded DNA (dsDNA). In contrast, we propose potential‐induced dsDNA dehybridization to distinguish fully matched target DNA (tDNA) from tDNA with a single nucleotide polymorphism (SNP) by following their dehybridization kinetics through in situ surface enhanced Raman scattering (SERS). The determination of the potential that evokes notable dehybridization of dsDNA without causing any desorption of the immobilized probe DNA (pDNA) from the electrode surface was performed by investigating the desorption kinetics of labelled single‐stranded DNA (ssDNA) and dehybridization kinetics of dsDNA with labelled tDNA. This comparatively simple approach allows for SNP detection within minutes.