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The Immobilization of Fructose Dehydrogenase on Nanoporous Gold Electrodes for the Detection of Fructose
Author(s) -
Siepenkoetter Till,
SalajKosla Urszula,
Magner Edmond
Publication year - 2017
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201600842
Subject(s) - nanoporous , fructose , biosensor , detection limit , chemistry , dehydrogenase , adsorption , electrode , thiol , linear range , chromatography , nuclear chemistry , combinatorial chemistry , enzyme , organic chemistry , biochemistry
Nanoporous gold electrodes were utilized as a support for the detection of d ‐fructose. The immobilization of fructose dehydrogenase was achieved by adsorption on thiol‐ and diazonium‐bound carboxylic acid functional groups and subsequent crosslinking through cabodiimide‐initiated amide bond formation. The biosensor showed a linear response in the range 0.05–0.3 mM of d ‐fructose with a sensitivity of 3.7±0.2 μA cm −2 mM −1 and a limit of detection of 1.2 μM. The response of the biosensor in a range of natural sweeteners and beverages compared very favorably to the results obtained by a commercially available kit. Accurate readings were obtainable after a very fast response time of less than 5 seconds. The biosensor showed high specificity towards d ‐fructose in the presence of interfering sugars.