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A Peptide‐Based Electrochemical Biosensor for Facile Measurement of Whole‐Blood Heparin
Author(s) -
Meng Fanyu,
Liang Weiguo,
Sun Haixuan,
Wu Ligang,
Gong Xiao,
Miao Peng
Publication year - 2017
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201600680
Subject(s) - dielectric spectroscopy , detection limit , biosensor , electrochemistry , heparin , electrode , materials science , linear range , peptide , electrochemical gas sensor , electrical impedance , chromatography , analytical chemistry (journal) , chemistry , nanotechnology , biochemistry , electrical engineering , engineering
The first peptide‐based electrochemical biosensor for the measurement of the anti‐coagulant heparin using electrochemical impedance spectroscopy is reported. The peptide immobilized on the electrode interface specially captures heparin with high affinity. The composites formed on the electrode can thus effectively repel electrochemical species, leading to an increase in interfacial charge transfer resistance. After analyzing the electrochemical signals, a linear detection range from 0.05 to 10.0 μg/mL is obtained. The detection limit is calculated to be 0.01 μg/mL, which is extremely low with such a simple sensing mechanism. Moreover, the proposed method has potential application for sensitive and separation‐free detection of heparin in whole‐blood samples.